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The Zebrafish Research Laboratory in Hacettepe University (HUZF) was established in 2015 within the Laboratory Animals Research and Application Center with the support of the Scientific and Technological Research Council of Turkey (TUBITAK, 214S174) to study the effects of mutations of rare disorders in Turkey. In 2017 laboratory infrastructure was improved and housing capacity was improved with another project by Hacettepe University Scientific Research Projects Coordination Unit (H.Ü.BAP, TAY-2017-12735).

The laboratory has legal and ethical permission from Republic Of Turkey Ministry Of Food, Agriculture And Livestock for breeding, supply and generating transgenic zebrafish lines by using genome editing tools.

The infrastructure holds three separate tank systems for housing of adult zebrafish. For newly arrived fish there is a separate quarantine unit and a nursery for larva. 1500 adult fish can be housed at full capacity. Systems are re-circulating through bio-filters, mechanical filters and UV-radiation and fully are quarantined.  Facility has its own reverse osmosis water purification system. Water quality values are monitored everyday and large-scale quality tests are carried out regularly.

Our research facility aims to help researchers to address their questions using zebrafish model.

HUZF has a fish room and an imaging room with stereomicroscope and a micro-injection system. The facility also has a separate quarantine system for newly arrived fish.

EQUIPMENT

-       2 custom made stand alone system and 1 Tecniplast active-blue system

-       Quarantine system

-       Micronjection system with micromanipulator

-       Stereomicroscope with camera attachment

-       2 incubators for embryos

-       Liquid nitrogen container for sperm cryopresevation

-       -20oC and -80oC freezers

-       PCR and electrophoresis system

-       Fluorometer

 

SERVICES

Gene knock out using genome editing techniques

Drug screening and toxicology tests in embryos